Sample Incubation
Patient serum is diluted and added to antigen-coated microwells. If the target autoantibody is present in the sample, it binds to the antigen on the well surface during incubation. Unbound antibodies are removed by washing.
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Advancing The Science Of
Enzyme-Linked Immunoassays
Always With Our RELISA® Test Kits
Our Process
How RELISA® Test Systems Deliver Consistency Across Every ELISA Run
RELISA® test kits follow a stepwise enzyme-linked immunosorbent assay process, where controlled incubation, detection, and measurement ensure consistent performance and dependable quantitative results across every run.
Detection
An enzyme-labeled secondary antibody is added, binding to any patient autoantibodies captured in Step 1. For isotype-specific detection, such as IgG and IgM anticardiolipin antibodies, separate conjugates are used to determine each isotype independently. After a final wash, a substrate solution triggers a measurable color change, with intensity directly proportional to the concentration of autoantibody present.
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Reading and Reporting
The reaction is stopped, and absorbance is measured on a standard microplate reader. Results are expressed as calibrated antibody units, giving your laboratory a quantifiable result that goes beyond positive or negative.
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Our Solutions
Comprehensive RELISA® Test System Your Laboratory Needs: In One Portfolio
From screening to confirmation, every RELISA® test kit in our portfolio is produced to the same uncompromising standard, with validated antigen substrates, matched reagent systems, and calibrated controls in every box.
RELISA® ANA Screen
A broad-spectrum ELISA immunoassay for the qualitative detection of antinuclear antibodies in human serum. Designed to screen for clinically significant autoantibodies, including SS-A/Ro, SS-B/La, Sm, Sm/RNP, dsDNA, and Scl-70 in patients with suspected systemic rheumatic disease. Positive results are recommended for reflex testing using HEp-2 or HEp-2000® slide assays for ANA pattern determination.
RELISA® ENA Single-Well Screen
A cost-effective single-well ELISA test kit for high-throughput ENA screening. Each microwell is coated with six of the most clinically significant extractable nuclear antigens — Sm, RNP, SS-A, SS-B, Scl-70, and Jo-1 simultaneously, allowing laboratories to efficiently identify and exclude ENA-negative samples before committing to specific autoantibody confirmation testing.
RELISA® ENA Multiparameter
A multiparameter ELISA immunoassay for the simultaneous semi-quantitative detection of six clinically significant autoantibody specificities: SS-A/Ro, SS-B/La, Sm, Sm/RNP, Scl-70, and Jo-1, in a single run. Provides an integrated quality control mechanism that validates procedural integrity without additional reagents.
RELISA® MPO / PR3
A quantitative ELISA test kit for the confirmatory detection of MPO-ANCA and PR3-ANCA autoantibodies in human serum. Provides laboratories with a validated enzyme immunoassay method for ANCA specificity confirmation following indirect immunofluorescence screening in the diagnostic workup of ANCA-associated vasculitis.
RELISA® Cardiolipin IgG & IgM
An isotype-specific ELISA immunoassay for the individual quantitative determination of IgG and IgM anticardiolipin antibodies in human serum. Separate isotype-specific conjugates are provided for independent determination of IgG and IgM, supporting the serological evaluation of antiphospholipid syndrome in patients with SLE or lupus-like syndromes.
Why Choose Us
Why Laboratories Choose RELISA® Test Kits
Immuno Concepts engineers RELISA® assays for laboratories that cannot afford variability. Every antigen substrate, every conjugate, every control is validated together, because a reliable result depends on every component performing as one.
Quantifiable Results
RELISA® assays deliver calibrated antibody unit values, giving labs the data needed for differential diagnosis, disease monitoring, and treatment assessment.
Validated Antigen Substrates
Every RELISA® microwell is coated with stabilised, well-characterised antigens produced in-house, ensuring consistent antibody capture and reproducible optical density performance across every lot.
Built-In Quality Control
Quality checks are integrated into our multiparameter kit, thereby validating procedural integrity at the strip level without requiring additional external controls or reagents.
Broad Clinical Coverage
From ANA screening to specific autoantibody confirmation across SLE, Sjögren's syndrome, systemic sclerosis, antiphospholipid syndrome, vasculitis, and other autoimmune diseases — one portfolio covers every application.
Complete Ready-To-Use Systems
Every RELISA® test kit ships with everything your laboratory needs: antigen-coated microwells, conjugate, substrate, stopping reagent, calibrator, buffers, and controls, validated as one integrated system.
Our Commitment
Behind Every RELISA® Result Is A Team Behind You
Good science deserves good support. That is why every RELISA® test kit comes backed by rigorous validation, dedicated technical expertise, and a global network built around your laboratory's needs.
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Antigen Quality
Every RELISA® assay begins with in-house antigen qualification, where purity, stability, and coating consistency are controlled at the source.
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Lot Validation
Every lot is validated against reference sera before release. If it does not meet our thresholds, it does not ship.
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Kit Assembly
Matched reagents, calibrators, and controls are assembled and validated as one complete system.
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Laboratory Support
From onboarding to ongoing technical guidance, our team is with you at every step: training, troubleshooting, and configuring RELISA® to enhance your workflow.
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Global Access
Wherever your laboratory operates, our distribution network ensures you always have direct access to every RELISA® test kit and the support that comes with it.
Ready To Take Your Autoantibody Testing Further With RELISA®?
The right ELISA test kit does not just perform, it transforms the way your laboratory approaches autoantibody confirmation. Let our team show you what RELISA® can do.