ELISA

RELISA® DNA

Patients with systemic lupus erythematosus (SLE) may produce antibodies to a variety of nuclear antigens, but antibodies directed against Sm (Smith antigen) and double stranded DNA (dsDNA) show the highest correlation with disease (1). Antibodies directed against Sm demonstrate a speckled ANA staining pattern while antibodies directed against dsDNA generally demonstrate a homogeneous ANA staining pattern. Although low levels of anti-dsDNA antibodies may be present in the serum of patients with rheumatoid arthritis, Sjögren’s syndrome, progressive systemic sclerosis, dermatomyositis, discoid lupus erythematosus, and mixed connective tissue disease (2), high levels of dsDNA antibodies are seen almost exclusively in SLE. Antibodies against dsDNA are thought to be involved in the pathogenesis of the most severe variants of SLE when deposited as immune complexes (3). Antibodies to dsDNA occur in high titer, and, because they correlate with disease activity (4), their detection is important in the management of SLE patients.

Several assays are available for the detection of anti-dsDNA antibodies. The most commonly used methods include indirect immunofluorescence, radioimmunoassay, counterimmunoelectrophoresis, and immunodiffusion (5-8). The Immuno Concepts RELISA® anti-dsDNA test system is an enzyme immunoassay (EIA) method that detects serum antibodies to dsDNA.

• Total assay time of 90 minutes

Test system comes with:

• Antigen coated microtiter wells

• Positive and negative controls

• Options for single or multi-point calibration curve

• Sample diluent

• PBS

• Conjugate

• Substrate solution

• Stopping reagent

Ordering information:

7096-17    RELISA® dsDNA Test System      96 wells

*Available outside the USA only

RELISA® dsDNA Test System

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RELISA® ENA (extractable nuclear antigens)

The RELISA® ENA assays were designed with the proper sensitivity and specificity to help the clinician in the diagnosis of autoimmune disease. Sera from over 2,000 patients with rheumatic disease and 1,000 normal individuals were tested during the development of the RELISA® ENA Test System. Immuno Concepts compared the results to reference methods such as Ouchterlony immunodiffusion, counterimmunoelectrophoresis, other ELISA methods and Western blotting. To verify the clinical utility of the assay, the laboratory results were also compared to the clinical history and diagnosis of the patient.

• Affinity purified native mammalian antigens

• Specificity and sensitivity are designed to provide diagnostically meaningful results

• Liquid stable ready-to-use controls and conjugates

• Total assay time of 90 minutes

Test system comes with…

• Antigen coated microtiter wells

• Positive and negative controls

• Sample diluent

• PBS

• Conjugate

• Substrate solution

• Stopping reagent

 

RELISA® ENA Single Well Screening Kit

The RELISA® ENA Single Well Screening Kit is designed to screen patient samples for six of the most common autoantibodies. Each microtiter well is coated with Sm, RNP, SS-A, SS-B, Scl-70 and Jo-1 autoantigens. This allows the laboratory to cost effectively screen out the ENA negative patient samples prior to determining the ENA specificity on any of the company’s other RELISA® ENA Test Systems. The strips are color-coded for easy identification and feature break-away wells.

Ordering Information:
7096-10    96 wells per kit
7696-10    576 wells per kit

Other kit configurations are available upon request.

For a copy of the package insert, please click on the appropriate link below.

RELISA® ENA Single Well Screening Test
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RELISA® ENA Multiparameter Kit

The RELISA® ENA Multiparameter Kit is designed for testing patient samples for six of the most common autoantibodies. This kit features a unique quality control system. The built-in RPC (RELISA® Procedure Check) well in each strip gives the laboratory maximum flexibility in determining their quality control procedures.

Ordering Information:
7096-09    96 wells per kit
7696-09    576 wells per kit

Other kit configurations are available upon request.

For a copy of the package insert, please click on the appropriate link below.

RELISA® ENA Multiparameter Antibody Screening Test
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